EpiCypher是一家為表觀遺傳學(xué)和染色質(zhì)生物學(xué)研究提供高質(zhì)量試劑和工具的專業(yè)制造商。EpiCypher生產(chǎn)的在E. coli中表達(dá)的重組人單核小體(組蛋白H2A、H2B、H3和H4各2個;accession numbers:H2A-P04908;H2B-O60814;H3.1-P68431;H4-P62805)由601位處的147個DNA堿基對纏繞在組蛋白上構(gòu)成。組蛋白H2B(由專門的半合成方法產(chǎn)生)在120位處含有泛素賴氨酸。核小體是染色質(zhì)的基本單位，Lowary和Widom鑒定的601序列是一個含有147堿基對的序列，對組蛋白八聚體具有高親和力，可用于核小體組裝，并含有5'biotin-TEG基團。適合用作酶篩選分析或效應(yīng)蛋白結(jié)合實驗的底物。

[1] Lowary and Widom J. Mol. Biol. (1998). PMID: 9514715

產(chǎn)品詳情

標(biāo)簽: Biotinylated

保存溫度:Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.

產(chǎn)品形式: H2BK120ub dNuc (14.4 µg protein weight, 25 µg DNA + protein) in 22.5 µL 10 mM Tris HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. Molarity = 5.12 μM. MW = 216,985.9 Da.

驗證數(shù)據(jù)

	Figure 1: Western blot data Western Analysis of Nucleosome, Recombinant Human, H2BK120ub.  Top Panel: Unmodified H3 (Lane 1) and H2BK120ub containing nucleosomes  (Lane 2) were probed with an anti-H2BK120ub antibody and analyzed via ECL  readout. Only the H2BK120ub sample produced a detectable signal. Bottom  Panel: Detail from Coomassie stained gel showing unmodified nucleosomes  (Lane 1) and H2BK120ub nucleosomes (Lane 2).
	Figure 2: Mass spec data H2BK120ub protein analyzed by high  resolution mass spectrometry. Expected mass = 22,321.8 Da. Determined  mass = 22,320.3 Da.
	Figure 3: Protein gel data Coomassie stained PAGE gel of proteins in Nucleosome, Recombinant  Human, H2BK120ub (1 μg) to demonstrate the purity of the histones in the  preparation. Sizes of molecular weight markers and positions of the core  histones (H2A, H2BK120ub, H3.1, and H4) are indicated.
	Figure 4: DNA gel data Nucleosome, Recombinant Human, H2BK120ub dNucs resolved via native  PAGE and stained with ethidium bromide to visualize DNA. Lane 1: Free  DNA (100 ng). Lane 2: Intact H2BK120ub nucleosome (400 ng).

訂購詳情

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